Journal
MOLECULAR CELL
Volume 58, Issue 4, Pages 632-643Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2015.03.002
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Funding
- Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT)
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Convergent advances in optical imaging and genetic engineering have fueled the development of new technologies for biological visualization. Those technologies include genetically encoded indicators based on fluorescent proteins (FPs) for imaging ions, molecules, and enzymatic activities to spy on cells, as phrased by Roger Tsien, by sneaking into specific tissues, cell types, or subcellular compartments, and reporting on specific intracellular activities. Here we review the current range of unimolecular indicators whose working principle is the conversion of a protein conformational change into a fluorescence signal. Many of the indicators have been developed from fluorescence resonance energy transfer-and single-FP-based approaches.
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