Journal
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
Volume 76, Issue 4, Pages 973-977Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ijrobp.2009.11.038
Keywords
Genetically engineered mouse models of cancer; Lung cancer; Micro computed tomography; K-Ras; p53
Funding
- Howard Hughes Medical Institute
- American Cancer Society Institutional Research [K08 CA 114176, P50 CA86355, R24 CA92782, U24 CA 092782]
- NCI [5-U01-CA84306, P30-CA14051]
- RSNA
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Purpose: To image a genetically engineered mouse model of non small-cell lung cancer with micro computed tomography (micro-CT) to measure tumor response to radiation therapy. Methods and Materials: The Cre-loxP system was used to generate primary lung cancers in mice with mutation in K-ras alone or in combination with p53 mutation. Mice were serially imaged by micro-CT, and tumor volumes were determined. A comparison of tumor volume by micro-CT and tumor histology was performed. Tumor response to radiation therapy (15.5 Gy) was assessed with micro-CT. Results: The tumor volume measured with free-breathing micro-CT scans was greater than the volume calculated by histology. Nevertheless, this imaging approach demonstrated that lung cancers with mutant p53 grew more rapidly than lung tumors with wild-type p53 and also showed that radiation therapy increased the doubling time of p53 mutant lung cancers fivefold. Conclusions: Micro-CT is an effective tool to noninvasively measure the growth of primary lung cancers in genetically engineered mice and assess tumor response to radiation therapy. This imaging approach will be useful to study the radiation biology of lung cancer. (C) 2010 Elsevier Inc.
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