Journal
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Volume 85, Issue 9, Pages 732-746Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09553000902785791
Keywords
Biomarker; DNA repair; -H2AX; deconvolution; foci; confocal microscopy; normal tissues; ionising radiation
Funding
- National Cancer Institute of Canada [14246]
- National Institute of Allergies and Infectious Diseases [AI067733-03]
- Canadian Foundation for Innovation
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Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as -H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.
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