Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 26, Issue 24, Pages 4358-4372Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E15-08-0578
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Funding
- National Institutes of Health [GM60992, GM30626]
- Bushrod H. Campbell and Adah F. Hall Charity Fund
- Robert W. Booth Fund at the University of Massachusetts Medical School
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Drosophila sperm are unusual in that they do not require the intraflagellar transport (IFT) system for assembly of their flagella. In the mouse, the IFT proteins are very abundant in testis, but we here show that mature sperm are completely devoid of them, making the importance of IFT to mammalian sperm development unclear. To address this question, we characterized spermiogenesis and fertility in the Ift88(Tg737Rpw) mouse. This mouse has a hypomorphic mutation in the gene encoding the IFT88 subunit of the IFT particle. This mutation is highly disruptive to ciliary assembly in other organs. Ift88(-/-) mice are completely sterile. They produce -350-fold fewer sperm than wild-type mice, and the remaining sperm completely lack or have very short flagella. The short flagella rarely have axonemes but assemble ectopic microtubules and outer dense fibers and accumulate improperly assembled fibrous sheath proteins. Thus IFT is essential for the formation but not the maintenance of mammalian sperm flagella.
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