Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 26, Issue 21, Pages 3879-3891Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E15-07-0545
Keywords
-
Categories
Funding
- Public Health Service from the National Institute of General Medical Sciences [GM-55816, GM-54200]
- Caerus Foundation
- Scleroderma Foundation
- Mayo Foundation
Ask authors/readers for more resources
Transforming growth factor beta (TGF beta) is a pleiotropic protein secreted from essentially all cell types and primary tissues. While TGF beta's actions reflect the activity of a number of signaling networks, the primary mediator of TGF beta responses are the Smad proteins. Following receptor activation, these cytoplasmic proteins form hetero-oligomeric complexes that translocate to the nucleus and affect gene transcription. Here, through biological, biochemical, and immunofluorescence approaches, sorting nexin 9 (SNX9) is identified as being required for Smad3-dependent responses. SNX9 interacts with phosphorylated (p) Smad3 independent of Smad2 or Smad4 and promotes more rapid nuclear delivery than that observed independent of ligand. Although SNX9 does not bind nucleoporins Nup153 or Nup214 or some beta importins (Imp7 or Imp beta), it mediates the association of pSmad3 with Imp8 and the nuclear membrane. This facilitates nuclear translocation of pSmad3 but not SNX9.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available