Journal
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
Volume 13, Issue -, Pages 77-89Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15226514.2011.568537
Keywords
protein engineering; directed evolution; remediation; enzymes; plants; microbes
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The combination of rational protein engineering and directed evolution techniques allow for the redesign of enzymes with tailored properties for use in environmental remediation. This review summarizes current molecular methods for either altering or improving protein function and highlights examples of how these methods can address bioremediation problems. Although much of the protein engineering applied to environmental clean-up employs microbial systems, there is great potential for and significant challenges to translating these approaches to plant systems for phytoremediation purposes. Protein engineering technologies combined with genomic information and metabolic engineering strategies hold promise for the design of plants and microbes to remediate organic and inorganic pollutants.
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