Investigation of a nanosuspension stabilized by Soluplus® to improve bioavailability

The purpose of this work was to explore the feasibility of using Soluplus (R) in preparing a fenofibrate (FBT) nanosuspension adopting wet media milling technology. HPMC and Soluplus (R) were used as stabilizers to prepare FBT/HPMC nanosuspension (F1) and FBT/Soluplus (R) nanosuspension (F2), respectively. The nanosuspensions were subjected to evaluations involving particle size, dissolution, preliminary stability and pharmacokinetic behavior. A marked reduction in particle size was achieved by nanosuspensions (from 17.55 mm to 642 nm (F1) and 344 nm (F2)). The nanosuspensions displayed almost complete dissolution while percentages of 30% and 13% were obtained by physical mixtures and coarse FBT separately. Soluplus (R) could stabilize the nanosuspension more effectively due to a weaker Ostwald ripening effect resulting from a slower diffusion of micelles formed by Soluplus (R) entrapping dissolved FBT than FBT exposed to pure water directly. In the in vivo evaluation, larger AUC(0-72 h) and C-max, and shorter T-max were obtained by the nanosuspensions. Significant differences were observed between the physical mixtures. The phenomenon of double peaks was present in this study. The major factor may be the multiple absorption sites of FBT. The current work indicated that Soluplus (R) is well suited for preparation of a nanosuspension with good stability and improved dissolution and bioavailability. (C) 2014 Elsevier B.V. All rights reserved.