Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 441, Issue 1-2, Pages 146-150Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2012.12.004
Keywords
Exogenous DNA; Histone; Nucleosome; Intranuclear disposition; Expression efficiency
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Japan Society for the Promotion of Science
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Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo. (C) 2012 Elsevier B. V. All rights reserved.
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