Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 436, Issue 1-2, Pages 240-247Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2012.06.043
Keywords
Carboxymethyl chitosan; 6-Mercaptopurine; GSH-dependent release; Polymeric prodrug
Categories
Funding
- National Natural Science Foundation of China [50973088]
- Natural Science Foundation of Hubei Province [2010CDA040]
- Chen Guang project of Wuhan [201140431092]
- Fundamental Research Funds for the Central Universities [2010-IV-009]
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In this work, an amphiphilic polymeric prodrug Cis-3-(9H-purin-6-ylthio)-acrylic acid-graft-carboxymethyl chitosan (PTA-g-CMCS) was designed and synthesized. In aqueous solution, this grafted polymer can self-assemble into spherical micelles with a size ranging from 104 to 285 nm and zeta potential ranging from -12.3 to -20.1 mV. For the release study, less than 24% of 6-Mercaptopurine (6-MP) was released from PTA-g-CMCS1 in the media containing 2 and 100 mu M glutathione (GSH), whereas 37%, 54% and 75% of 6-MP was released from the media with GSH of 1, 2 and 10 mM, respectively. Besides, pH and drug content of the polymeric prodrug only presented slight influence on the 6-MP release. MTT assay demonstrated that this system had higher inhibition ratio on HL-60 cells (human promyelocytic leukemia cells) in the presence of GSH and lower cytotoxicity on mouse fibroblast cell line (L929). Therefore, this nano-sized system is glutathione-dependent, and it can be employed as a potential carrier for the controlled release of 6-MP. (c) 2012 Elsevier B.V. All rights reserved.
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