Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 417, Issue 1-2, Pages 70-82Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2010.12.010
Keywords
MALDI TOF mass spectrometry; Biopharmaceuticals; Collision-induced dissociation; PACA; PLGA; Protein/peptide-polymer conjugate
Categories
Ask authors/readers for more resources
The development and quality assessment of modern biopharmaceuticals, particularly protein and peptide drugs, requires an array of analytical techniques to assess the integrity of the bioactive molecule during formulation and administration. Mass spectrometry is one of these methods and is particularly suitable for determining chemical modifications of protein and peptide drugs. The emphasis of this review is the identification of covalent interactions between protein and peptide bioactives with polymeric pharmaceutical formulations using mass spectrometry with the main focus on matrix-assisted laser desorption/ionization (MALDI) coupled tandem time-of-flight (TOF/TOF) mass spectrometry (MS). The basics of MALDI TOF MS and collision-induced dissociation (CID)-based ion fragmentation will be explained and applications for qualitative characterization of protein and peptide drugs and their interactions with pharmaceutical polymers will be discussed using three case studies. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available