Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 406, Issue 1-2, Pages 128-134Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2010.12.023
Keywords
Nanoparticles; Human serum albumin (HSA); Folic acid; Folate receptor; Cancer targeting
Categories
Funding
- Hilfe fur krebskranke Kinder Frankfurt e.V. und der Frankfurter Stiftung fur krebskranke Kinder
Ask authors/readers for more resources
Folic acid has been previously demonstrated to mediate intracellular nanoparticle uptake. Here, we investigated cellular uptake of folic acid-conjugated human serum albumin nanoparticles (HSA NPs). HSA NPs were prepared by desolvation and stabilised by chemical cross-linking with glutaraldehyde. Folic acid was covalently coupled to amino groups on the surface of HSA NPs by carbodiimide reaction. Preparation resulted in spherical HSA NPs with diameters of 239 +/- 26 nm. As shown by size exclusion chromatography, 7.40 +/- 0.90 mu g folate was bound per mg HSA NPs. Cellular NP binding and uptake were studied in primary normal human foreskin fibroblasts (HFFs), the human neuroblastoma cell line UKF-NB-3, and the rat glioblastoma cell line 101/8 by fluorescence spectrophotometry, flow cytometry, and confocal laser scanning microscopy. Covalent conjugation of folic acid to HSA NPs increased NP uptake into cancer cells but not into HFFs. Free folic acid interfered with cancer cell uptake of folic acid-conjugated HSA NPs but not with uptake of folic acid-conjugated HSA NPs into HFFs. These data suggest that covalent linkage of folic acid can specifically increase cancer cell HSA NP uptake. (c) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available