Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 399, Issue C, Pages 95-102Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2014.07.022
Keywords
MicroRNA; miR-378; Ovary; Granulosa cell; Progesterone receptor
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Institutes for Health Research (CIHR)
- NSERC EmbryoGENE Research Network
- Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA)
- National Natural Science Foundation of China
- China Scholarship Council
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In developing ovarian follicles, the progesterone receptor (PGR) is essential for mediating transcription of key factors that coordinate cellular functions including follicular remodeling. With recent investigations examining the role of microRNA (miRNA) in regulating ovarian function we used a lentiviral approach to over express miR-378 in cultured primary porcine granulosa cells to study the role this miRNA may play in granulosa cell development. We revealed that miR-378-3p decreased protein levels and mRNA levels of PGR via targeting its 3'UTR. We observed that this regulation of PGR by miR-378-3p resulted in a corresponding decrease in gene transcripts of ADAMTS1, CTSL1, and PPARG, all known to be regulated by PGR and important for follicular maturation and remodeling. Our study provides the first evidence for post-transcriptional regulation of PGR and further elucidates the role of miR-378-3p in the ovary. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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