Journal
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
Volume 19, Issue 3, Pages 231-237Publisher
SPRINGER
DOI: 10.1007/s10989-012-9340-x
Keywords
Whey protein; Trypsin; Antioxidant activity; Antihypertensive activity; Response surface method
Categories
Funding
- Indian Council of Agricultural Research, New Delhi, India
Ask authors/readers for more resources
Whey is a protein complex derived from milk, exhibit highest protein quality rating among other proteins, being touted as a functional food with number of health benefits. In the present investigation, whey proteins hydrolysates produced using trypsin enzyme to augment antioxidant activity and to assess angiotensin converting enzyme (ACE) inhibition activity. Hydrolysis parameters were standardized applying response surface methodology. The response antioxidant activity in terms of Trolox equivalent antioxidant capacity (TEAC) values was determined by radical scavenging assay method. Optimum conditions for maximum antioxidant activity were standardized at 88 A degrees C of preheating, 7.3 pH, 0.05 enzymes to substrate ratio and hydrolysis was carried up to 8 h at 36.5 A degrees C. Resulting peptide fractions obtained at 11.8 % of degree of hydrolysis displayed antioxidant capacity with TEAC values of 1.37 +/- A 0.12. The designed model found to be significant with R-2 value of 0.9972 for antioxidant activity and lack of fit test-as non significant, indicating that the optimized conditions were best suited. The hydrolysate further investigated for antihypertensive activity. The outcome indicate that to affect decrease in ACE inhibition activity 4,166.72 mu g of native whey protein is required when compared to 229.96 mu g of hydrolysates. These results indicate hydrolysate produced under these conditions could be an effective nutraceutical.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available