Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 414, Issue C, Pages 194-201Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2015.06.023
Keywords
Membrane progestin receptor alpha; Progestin; Sperm motility; Atlantic croaker; Epidermal growth factor receptor; EGFR; ErbB2
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Funding
- Agriculture and Food Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2009-65203-05757]
- NIFA [581574, 2009-65203-05757] Funding Source: Federal RePORTER
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The intracellular pathways mediating rapid, nongenomic progestin stimulation of sperm motility remain unclear. The role of epidermal growth factor receptors (Egfr and ErbB2) and mitogen-activated protein kinase (Mapk) in membrane progestin receptor-alpha (mPR alpha)-mediated progestin stimulation of sperm hypermotility was examined in a teleost, Atlantic croaker. Inhibition of upstream regulators of Egfr, intracellular tyrosine kinase (Src) with PP2, and matrix metalloproteinase (MMP) with Ilomastat, abolished progestin-initiated sperm hypermotility by 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S; 20 nM) and a specific mPR alpha agonist, Org OD 02-0 (20 nM). Pretreatment of croaker sperm with EGFR inhibitors, AG1478 (5 mu M) and RG13022 (50 mu M), the ErbB2 inhibitor, AG879 (5 nM), or the MEK1/2 inhibitor, U0126 (500 nM) blocked progestin stimulation of sperm motility. Levels of phosphorylated extracellular-related kinase 1 and 2 (P-Erk1/2) were increased after 20 beta-S treatment. These results demonstrate that progestin-mediated hypermotility via mPR alpha in croaker sperm involves activation of the Egfr, ErbB2 and Mapk pathways. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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