4.5 Article

Coordination of RNA Polymerase II Pausing and 3 ' End Processing Factor Recruitment with Alternative Polyadenylation

Journal

MOLECULAR AND CELLULAR BIOLOGY
Volume 36, Issue 2, Pages 295-303

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00898-15

Keywords

-

Funding

  1. HHS \ NIH \ National Institute of General Medical Sciences (NIGMS) [GM58613]
  2. NSF \ BIO \ Division of Molecular and Cellular Biosciences (MCB) [MCB-0919099]
  3. NATIONAL CANCER INSTITUTE [R01CA170859] Funding Source: NIH RePORTER
  4. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM058613] Funding Source: NIH RePORTER

Ask authors/readers for more resources

Most mammalian genes produce transcripts whose 3' ends are processed at multiple alternative positions by cleavage/polyadenylation (CPA). Poly(A) site cleavage frequently occurs cotranscriptionally and is facilitated by CPA factor binding to the RNA polymerase II (Pol II) C-terminal domain (CTD) phosphorylated on Ser2 residues of its heptad repeats (YS2PTSPS). The function of cotranscriptional events in the selection of alternative poly(A) sites is poorly understood. We investigated Pol II pausing, CTD Ser2 phosphorylation, and processing factor CstF recruitment at wild-type and mutant IgM transgenes that use alternative poly(A) sites to produce mRNAs encoding the secreted and membrane-bound forms of the immunoglobulin (Ig) heavy chain. The results show that the sites of Pol II pausing and processing factor recruitment change depending on which poly(A) site is utilized. In contrast, the extent of Pol II CTD Ser2 phosphorylation does not closely correlate with poly(A) site selection. We conclude that changes in properties of the transcription elongation complex closely correlate with utilization of different poly(A) sites, suggesting that cotranscriptional events may influence the decision between alternative modes of pre-mRNA 3' end processing.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available