Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 35, Issue 19, Pages 3370-3380Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00509-15
Keywords
-
Categories
Funding
- Fondation ARC [PJA 20141201647]
- Agence Nationale de la Recherche
- Institut National du Cancer [PLBIO15-134]
- Ligue Contre le Cancer-Comite de Haute-Garonne
- Canceropole Grand-Sud-Ouest
Ask authors/readers for more resources
Eukaryotic gene expression is often controlled by distant regulatory elements. In developing B lymphocytes, transcription is associated with V(D) J recombination at immunoglobulin loci. This process is regulated by remote cis-acting elements. At the immunoglobulin heavy chain (IgH) locus, the 3' regulatory region (3' RR) promotes transcription in mature B cells. This led to the notion that the 3' RR orchestrates the IgH locus activity at late stages of B cell maturation only. However, long-range interactions involving the 3' RR were detected in early B cells, but the functional consequences of these interactions were unknown. Here we show that not only does the 3' RR affect transcription at distant sites within the IgH variable region but also it conveys a transcriptional silencing activity on both sense and antisense transcription. The 3' RR-mediated silencing activity is switched off upon completion of V-H-DJ(H) recombination. Our findings reveal a developmentally controlled, stage-dependent shift in the transcriptional activity of a master regulatory element.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available