Journal
MOLECULAR & CELLULAR TOXICOLOGY
Volume 11, Issue 3, Pages 323-334Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
DOI: 10.1007/s13273-015-0032-4
Keywords
Persistent organic pollutants (POPs); Toxaphene (TOX); Chlordane (CD); Transcriptomics; Gene Ontology (GO); Hepatotoxicity
Categories
Funding
- Korea Research Foundation Grants from Korea Ministry of Environment [2014001650003]
- KIST Program of the Republic of Korea
Ask authors/readers for more resources
Determination of gene expression profile alterations following dose-dependent exposure to toxicants will greatly improve the utility of arrays in identifying biomarkers and elucidating modes of toxicity. In the present study, the effects of increasing doses of persistent organic pollutants (POPs) on the expression of toxicity-related genes were evaluated. Human hepatocellular carcinoma (HepG2) cells were exposed to nontoxic (NT) and 20% inhibitory concentrations (IC20) of the well-known toxic PoP compounds, toxaphene (TOX) and chlordane (CD), for 48 h. Transcriptomic profiling showed differential gene expression patterns and a profound effect on gene expression changes in the IC20 exposure group. Through clustering analysis, 98 NT- and 336 IC20-specific genes and 73 genes expressed dose-dependently were identified. Gene Ontology (GO) analysis identified several key pathways related to the adverse health effects of POPs. In conclusion, this report describes changes in gene expression profiles in hepatocytes in response to different doses of POPs and relates these changes to hepatotoxicity-related pathways. Moreover, novel genes and pathways that may potentially play a role in the prevention of liver disease due to POP exposure were identified.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available