Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 14, Issue 4, Pages 841-853Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M114.044222
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Funding
- Federation of European Biochemical Societies (FEBS)
- Max-Planck Society for the Advancement of Science
- Novo Nordisk Foundation Center for Basic Metabolic Research Copenhagen, Denmark
- Commission's 7th Framework Programme [HEALTH-F4-2008-201648/PROSPECTS]
- NNF Center for Basic Metabolic Research [Treebak Group] Funding Source: researchfish
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Skeletal muscle constitutes 40% of individual body mass and plays vital roles in locomotion and whole-body metabolism. Proteomics of skeletal muscle is challenging because of highly abundant contractile proteins that interfere with detection of regulatory proteins. Using a state-of-the art MS workflow and a strategy to map identifications from the C2C12 cell line model to tissues, we identified a total of 10,218 proteins, including skeletal muscle specific transcription factors like myod1 and myogenin and circadian clock proteins. We obtain absolute abundances for proteins expressed in a muscle cell line and skeletal muscle, which should serve as a valuable resource. Quantitation of protein isoforms of glucose uptake signaling pathways and in glucose and lipid metabolic pathways provides a detailed metabolic map of the cell line compared with tissue. This revealed unexpectedly complex regulation of AMP-activated protein kinase and insulin signaling in muscle tissue at the level of enzyme isoforms.
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