Journal
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume 9, Issue -, Pages 1601-1615Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S58334
Keywords
superparamagnetic iron oxide nanoparticles; BSA; bimodal imaging; MRI; targeted imaging
Funding
- National Natural Science Foundation of China [81371618, 81171393, 81271629]
- Key Basic Research Project of Shanghai Science and Technology [10JC1412900]
- Shanghai Innovation program [14ZZ039]
- Nanotechnology Program of Shanghai Science and Technology Committee [11nm0504500, 12nm0500800]
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Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma -diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA.SPIONs). The BSA.SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r(1), r(2)) values of the BSA.SPIONs were determined to be 11.6 and 154.2 s(-1) per mM of Fe3+ respectively. The r(2)/r(1) ratio of 13.3 ensured its application as the T-2-weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the (dye)BSA.SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis.
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