Journal
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume 7, Issue -, Pages 3365-3378Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S32264
Keywords
magnetic therapy; magnetic nanoparticles; bone repair; magnetic responsive
Funding
- National Basic Research Program of China (National 973 program) [2011CB606206, 2012CB619103]
- National Natural Science Foundation of China [31070849, 51133004]
- Department of Science and Technology of Sichuan Province [2009HH0001, 2009SZ0137]
- Ministry of Science and Technology [2010DFA51550]
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Introduction: In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group. Aim and methods: In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests. Results: The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation. Conclusion: The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.
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