Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 15, Issue 9, Pages 15845-15857Publisher
MDPI
DOI: 10.3390/ijms150915845
Keywords
miR-320a; alpha-synuclein aggregation; Hsc 70; chaperone-mediated autophagy; Parkinson disease
Funding
- Affiliated Hospital of Qingdao University
Ask authors/readers for more resources
Chaperone-mediated autophagy (CMA) is involved in wild-type alpha-synuclein degradation in Parkinson's disease (PD), and LAMP2A and Hsc 70 have recently been indicated to be deregulated by microRNAs. To recognize the regularory role of miR-320a in CMA and the possible role in alpha-synuclein degradation, in the present study, we examined the targeting and regulating role of miR-320 in Hsc 70 expression. We first constructed an alpha-synuclein-overexpressed human neuroblastoma cell line, SH-SY5Y-Syn(+), stably over-expressing wild-type alpha-synuclein and sensitive to an autophagy inhibitor, which exerted no effect on the expression of LAMP2A and Hsc 70. Then we evaluated the influence on the CMA by miR-320a in the SH-SY5Y-Syn(+) cells. It was shown that miR-320a mimics transfection of specifically targeted Hsc 70 and reduced its expression at both mRNA and protein levels, however, the other key CMA molecule, LAMP2A was not regulated by miR-320a. Further, the reduced Hsc 70 attenuated the alpha-synuclein degradation in the SH-SY5Y-Syn(+) cells, and induced a significantly high level of alpha-synuclein accumulation. In conclusion, we demonstrate that miR-320a specifically targeted the 3' UTR of Hsc 70, decreased Hsc 70 expression at both protein and mRNA levels in alpha-synuclein-over-expressed SH-SY5Y cells, and resulted in significant alpha-synuclein intracellular accumulation. These results imply that miR-320a might be implicated in the alpha-synuclein aggravation in PD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available