Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 15, Issue 5, Pages 8821-8834Publisher
MDPI
DOI: 10.3390/ijms15058821
Keywords
high gradient magnetic separation; bead-cell binding; iron content; paramagnetic nanoparticles; magnetic labeling; cell separation
Funding
- Thailand Research Fund [BRG53_0052]
- Faculty of Graduate Studies, Mahidol University, Bangkok, Thailand
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Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 - exp(-kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding.
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