Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 15, Issue 9, Pages 16787-16799Publisher
MDPI AG
DOI: 10.3390/ijms150916787
Keywords
IDE; neuropeptide; metalloprotease; cryptome; mass spectrometry; surface plasmon resonance
Funding
- FIRB RENAME [RBAP114AMK, PRIN 2008R23Z7K]
- EuroNanoMed META [5/EuroNanoMed/2012, 2012/07/B/NZ4/01478]
- Polish National Centre for Science
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The term cryptome refers to the subset of cryptic peptides with bioactivities that are often unpredictable and very different from the parent protein. These cryptic peptides are generated by proteolytic cleavage of proteases, whose identification in vivo can be very challenging. In this work, we show that insulin-degrading enzyme (IDE) is able to degrade specific amino acid sequences present in the neuropeptide pro-NPFFA (NPFF precursor), generating some cryptic peptides that are also observed after incubation with rat brain cortex homogenate. The reported experimental findings support the increasingly accredited hypothesis, according to which, due to its wide substrate selectivity, IDE is involved in a wide variety of physiopathological processes.
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