Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 14, Issue 1, Pages 1952-1963Publisher
MDPI
DOI: 10.3390/ijms14011952
Keywords
FAD; autofluorescence; living cell; fluorescence lifetime imaging; intracellular pH; fluorescence decay
Funding
- Ministry of Education, Culture, Sports, Science and Technology in Japan [20245001, 2306]
- Grants-in-Aid for Scientific Research [24107501] Funding Source: KAKEN
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We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
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