Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 14, Issue 9, Pages 19276-19293Publisher
MDPI
DOI: 10.3390/ijms140919276
Keywords
magnetic force bioreactor; tissue engineering; mechanotransduction; human mesenchymal stem cells; osteogenic differentiation
Funding
- EU FP7 [EXPERTISSUES NMP3-CT-2004-500328]
- EPSRC [EP/H028277/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H028277/1] Funding Source: researchfish
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Mechanical cues are employed to promote stem cell differentiation and functional tissue formation in tissue engineering and regenerative medicine. We have developed a Magnetic Force Bioreactor (MFB) that delivers highly targeted local forces to cells at a pico-newton level, utilizing magnetic micro- and nano-particles to target cell surface receptors. In this study, we investigated the effects of magnetically targeting and actuating specific two mechanical-sensitive cell membrane receptorsplatelet-derived growth factor receptor (PDGFR) and integrin (3). It was found that a higher mineral-to-matrix ratio was obtained after three weeks of magneto-mechanical stimulation coupled with osteogenic medium culture by initially targeting PDGFR compared with targeting integrin (3) and non-treated controls. Moreover, different initiation sites caused a differentiated response profile when using a 2-day-lagged magneto-mechanical stimulation over culture periods of 7 and 12 days). However, both resulted in statistically higher osteogenic marker genes expression compared with immediate magneto-mechanical stimulation. These results provide insights into important parameters for designing appropriate protocols for ex vivo induced bone formation via magneto-mechanical actuation.
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