Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 14, Issue 12, Pages 24560-24580Publisher
MDPI
DOI: 10.3390/ijms141224560
Keywords
MALDI mass spectrometry imaging; FTICR mass spectrometry; lipidomics; hypoxia; necrosis; cancer
Funding
- FRS-FNRS Televie
- Fonds de la Recherche Scientifique-FNRS (F.R.S.-FNRS, Belgium)
- Foundation against Cancer (Belgium)
- Centre Anticancereux de l'Universite de Liege
- REFRACT project (Actions de Recherche Concertees, Belgium)
- PAI (Belgium)
- Televie-FNRS grant
- University of Liege research council under the REFRACT project
- European Funds for regional development (FEDER)
- FNRS
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Many studies have evidenced the main role of lipids in physiological and also pathological processes such as cancer, diabetes or neurodegenerative diseases. The identification and the in situ localization of specific low-abundant lipid species involved in cancer biology are still challenging for both fundamental studies and lipid marker discovery. In this paper, we report the identification and the localization of specific isobaric minor phospholipids in human breast cancer xenografts by FTICR MALDI imaging supported by histochemistry. These potential candidates can be further confirmed by liquid chromatography coupled with electrospray mass spectrometry (LC-ESI-MS) after extraction from the region of interest defined by MALDI imaging. Finally, this study highlights the importance of characterizing the heterogeneous distribution of low-abundant lipid species, relevant in complex histological samples for biological purposes.
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