Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 14, Issue 1, Pages 17-29Publisher
MDPI AG
DOI: 10.3390/ijms14010017
Keywords
polyamine microsphere; characterization; selective adsorption; protein; lipase
Funding
- National High Technology Research and Development Program of China (863 Program) [2006AA10Z335]
- Fundamental Research Funds for the Central Universities [JUSRP211A09]
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A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation of scanning electron microscopy showed that the polyamine microsphere is a regular ball with a smooth surface. The diameter distribution of the microsphere is 0.37-4.29 mu m. The isoelectric point of the microsphere is 10.6. The microsphere can adsorb proteins through the co-effect of electrostatic and hydrophobic interactions. Among the proteins tested, the highest value of adsorption of microsphere, 127.8 mg.g(-1) microsphere, was obtained with lipase. In comparison with other proteins, the hydrophobic force is more important in promoting the adsorption of lipase. The microsphere can preferentially adsorb lipase from an even mixture of proteins. The optimum temperature and pH for the selective adsorption of lipase by the microsphere was 35 degrees C and pH 7.0.
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