Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 13, Issue 8, Pages 10537-10552Publisher
MDPI AG
DOI: 10.3390/ijms130810537
Keywords
X-ray crystallography; aptamer; interaction; RNA/DNA-protein complex
Funding
- Netherlands Organisation for Scientific Research
- Netherlands Institute for Space Research [ALW-GO-PL/08-08]
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Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms. While reports of new aptamers are numerous, characterization of their specific interaction is often restricted to the affinity of binding (K-D). Over the years, crystal structures of aptamer-protein complexes have only scarcely become available. Here we describe some relevant technical issues about the process of crystallizing aptamer-protein complexes and highlight some biochemical details on the molecular basis of selected aptamer-protein interactions. In addition, alternative experimental and computational approaches are discussed to study aptamer-protein interactions.
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