Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 12, Issue 7, Pages 4522-4535Publisher
MDPI AG
DOI: 10.3390/ijms12074522
Keywords
chiral thiourea compounds; tobacco mosaic virus; antiviral activity; mechanism of action
Funding
- National Key Project for Basic Research [2010CB126105, 2010CB134504]
- National Key Technologies R & D Program of China [2011BAE06B05-6]
- National Natural Science Foundation [20872021]
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Using half-leaf method O,O'-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3-phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 mu g/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 mu g/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K-326 (Nicotiana tabacum K-326) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.
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