Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 11, Issue 1, Pages 173-188Publisher
MDPI
DOI: 10.3390/ijms11010173
Keywords
fluorescence correlation spectroscopy; host-guest chemistry; adamantane; cyclodextrin
Funding
- Ministerio de Ciencia e Innovacion
- Xunta de Galicia [CTQ2004-07683-C02-02, INCITE07PXI209034ES, INCITE08E1R209060ES]
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The host-guest complexation between an Alexa 488 labelled adamantane derivative and beta-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1: 1 complex stoichiometry and a high association equilibrium constant of K = 5.2 x 10(4) M-1 are obtained in aqueous solution at 25 degrees C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
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