Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 10, Issue 4, Pages 1788-1807Publisher
MDPI
DOI: 10.3390/ijms10041788
Keywords
RNA; non-enzymatic recombination; non-enzymatic ligation; RNA bulge loops; RNA internal loops; RNA world; origin of life
Funding
- Russian Academy of Sciences
- program for support of leading scientific schools [NSh-3689.2008.4]
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RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the RNA world, which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of catalytic roles necessary in primitive self-replicating systems. Here we report on the study of recombination reaction occuring between two 96 nucleotides (nts) fragments of RNAs under physiological conditions and governed by a short oligodeoxyribonucleotide template, partially complementary to sequences within each of the RNAs. Analysis of recombination products shows that ligation is predominantly template-directed, and occurs within the complementary complex with the template in butt-to-butt manner, in 1- or 3- nts bulges or in 2-3 nts internal loops. Minor recombination products formed in the template-independent manner are detected as well.
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