Journal
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Volume 35, Issue 2, Pages 453-459Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2014.2028
Keywords
Schisandrae semen essential oil; oxidative stress; reactive oxygen species; apoptosis; nuclear factor erythroid-2-related factor 2/heme oxygenase-1
Categories
Funding
- MOTIE/KIAT [10040391]
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The aim of the present study was to examine the cytoprotective effects of Schisandrae semen essential oil (SSeo), purified from Schisandrae fructus, against oxidative stress-induced cell damage in C2C12 myoblasts. SSeo attenuated hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against the intracellular reactive oxygen species (ROS) that were induced by H2O2. SSeo also inhibited comet tail formation, chromatin condensation and phospho-histone gamma H2A.X expression, suggesting that it prevents H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, SSeo significantly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction of nuclear factor erythroid-2-related factor 2 (Nrf2) in a time- and concentration-dependent manner. In addition, the protective effect of SSeo on H2O2-induced C2C12 cell damage was significantly inhibited by zinc protoporphyrin IX, an HO-1 competitive inhibitor, in C2C12 cells. These findings suggest that SSeo augments the cellular antioxidant defense capacity through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway, thereby protecting the C2C12 cells from H2O2-induced oxidative cytotoxicity. As a result, SSeo may have therapeutic potential in the development of functional foods and as the raw material for medicines to protect against oxidative stress.
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