Journal
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
Volume 10, Issue 3, Pages 286-291Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijms.5343
Keywords
kupffer cells; emulsified isoflurane; oxidative stress; rats; apoptosis
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Funding
- Shanghai Municipal Education Committee [12ZZ081]
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Objective: To investigate the protective effect of emulsified isoflurane (EI) preconditioning on isolated rat Kupffer cells (KCs) subjected to hypoxia/reoxygenation (H/R)-induced injury. Materials and methods: KCs were isolated by collagenase digestion and purified by Percoll density gradient centrifugation. Primary cultured KCs were divided into five groups: control, H/R plus 0.1% lipid preconditioning, and H/R plus 0.05%, 0.1% or 0.2% emulsified isoflurane preconditioning groups. H/R was induced by 4 h of hypoxia followed by 6 h of reoxygenation. Reactive oxygen species (ROS) production in the KCs and the concentration of tumor necrosis factor-a (TNF-alpha) in the KC culture media were measured, and the apoptosis of KCs was assayed concomitantly. Results: ROS and TNF-alpha production were markedly induced in the H/R + lipid group, and lower in the 0.2% and 0.1% EI groups (P<0.05). The apoptotic rate in the H/R + lipid group was significantly higher than that in the 0.2% and 0.1% EI groups (P<0.05). Conclusions: Emulsified isoflurane protects isolated rat KCs against H/R induced injury by decreasing the production of ROS and TNF-alpha and attenuating apoptosis in KCs.
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