Journal
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
Volume 302, Issue 1, Pages 4-9Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ijmm.2011.07.003
Keywords
Enterotoxigenic Escherichia coli (ETEC); Virulence and antibiotic resistance plasmid; Sequence analysis; Toxin-specific locus (TSL)
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Funding
- German-Hungarian Fellowship
- European Community [LSHB-CT-2005-12061]
- Hungarian Academy of Sciences
- Hungarian Basic Science Fund (OTKA) [68347]
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In this study the plasmid pTC, a 90 kb self-conjugative virulence plasmid of the porcine enterotoxigenic Escherichia coli (ETEC) strain EC2173 encoding the STa and STb heat-stable enterotoxins and tetracycline resistance, has been sequenced in two steps. As a result we identified five main distinct regions of pTC: (i) the maintenance region responsible for the extreme stability of the plasmid, (ii) the TSL (toxin-specific locus comprising the estA and estB genes) which is unique and characteristic for pTC, (iii) a Tn10 transposon, encoding tetracycline resistance, (iv) the tra (plasmid transfer) region, and (v) the colE1-like origin of replication. It is concluded that pTC is a self-transmissible composite plasmid harbouring antibiotic resistance and virulence genes. pTC belongs to a group of large conjugative E. coli plasmids represented by NR1 with a widespread tra backbone which might have evolved from a common ancestor. This is the first report of a completely sequenced animal ETEC virulence plasmid containing an antimicrobial resistance locus, thereby representing a selection advantage for spread of pathogenicity in the presence of antimicrobials leading to increased disease potential. (C) 2011 Published by Elsevier GmbH.
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