Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 354, Issue -, Pages 54-61Publisher
ELSEVIER
DOI: 10.1016/j.ijms.2013.05.016
Keywords
RNA/DNA pyrimidine nucleosides; Protomers; IRMPD spectroscopy; Conformational preference; Ab initio MD simulations
Funding
- Ministero dell'Istruzione dell'Universita e della Ricerca [RBPRO5NWWC_003]
- European Community's Seventh Framework Programme [226716]
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The ESI-formed protonated 2'-deoxycytidine, cytidine, cytarabine, and gemcitabine have been probed using infrared multiphoton dissociation (IRMPD) spectroscopy performed in the 900-2000 cm(-1) region at CLIO, the Orsay Free Electron Laser facility, and in the 2800-3800 cm(-1) region using a YAG-laser coupled to a table-top optical parametric oscillator/amplifier (OPO/OPA). The IRMPD spectra are compared of the protonated nucleosides with the IR spectra of their B3LYP/6-311++G(d,p)-optimized isomeric forms. The stability at room temperature of some conformers has been investigated by means of ab initio molecular dynamics simulations. The IRMPD spectra are consistent with the formation in the ESI source of both the N3- and the O2-protonated nucleosides. The most favoured members of both families are characterized by the pyrimidine base oriented anti to the furanose moiety. Concerning the O2-protonated nucleosides, IRMPD spectra and thermochemical considerations support the predominant formation of the structures with the proton oriented up relative to the furanose moiety. (C) 2013 Elsevier B.V. All rights reserved.
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