Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 312, Issue -, Pages 114-121Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2011.06.003
Keywords
ADP-ribosylated peptide; Mono ADP-ribosylation; Poly ADP-ribosylation; Fragmentation pattern; Tandem mass spectrometry
Funding
- NIH [T32 GM07750]
- National Center for Research Resources [1S10RR-017262-01]
- University of Washington's Proteomics Resource [UWPR95794]
- [NIH 1U54 AI57141-02]
- [NIH AI064515]
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The use of tandem mass spectrometry to identify and characterize sites of protein adenosine diphosphate (ADP) ribosylation will be reviewed. Specifically, we will focus on data acquisition schemes and fragmentation techniques that provide peptide sequence and modification site information. Also discussed are uses of synthetic standards to aid characterization, and an enzymatic method that converts ADP-ribosylated peptides into ribosyl mono phosphorylated peptides making identification amenable to traditional phosphopeptide characterization methods. Finally the potential uses of these techniques to characterize poly ADP-ribosylation sites, and inherent challenges, are addressed. (C) 2011 Elsevier B.V. All rights reserved.
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