Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 304, Issue 2-3, Pages 172-183Publisher
ELSEVIER
DOI: 10.1016/j.ijms.2010.10.003
Keywords
Circulating cell free DNA; Electrospray ionization mass spectrometry (ESI-MS); Matrix assisted laser desorption ionization mass spectrometry (MALDI-MS); Short oligonucleotide mass analysis (SOMA); Polymerase chain reaction (RE-PCR)
Funding
- National Institutes of Health [1RO1 CA69390, 1RO1 CA112231]
- Barnett Institute [898]
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In the past decade, cell free DNA, or circulating cell free DNA, or cell free circulating DNA, isolated from body fluids such as plasma/serum/urine has emerged as an important tool for clinical diagnostics. The molecular biology of circulating cell free DNA is poorly understood but there is currently an increased effort to understand the origin, mechanism of its circulation, and sensitive characterization for the development of diagnostic applications. There has been considerable progress towards these goals using real-time polymerase chain reaction (rt-PCR) technique. More recently, new attempts to incorporate mass spectrometric techniques to develop accurate and highly sensitive high-throughput clinical diagnostic tests have been reported. This review focuses on the methods to isolate circulating cell free DNA from body fluids, their quantitative analysis and mass spectrometry based characterization in evolving applications as prenatal and cancer diagnostic tools. (C) 2010 Elsevier B.V. All rights reserved.
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