Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 305, Issue 2-3, Pages 131-137Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2010.10.017
Keywords
Glycosaminoglycan; Chondroitin sulfate; Dermatan sulfate; Tandem MS; Multistage MS; Electrospray MS
Funding
- NIH [P41RR10888, R01098950, S10RR020946]
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Chondroitin/dermatan sulfate (CS/DS) is a glycosaminoglycan (GAG) found in abundance in extracellular matrices. In connective tissue, CS/DS proteoglycans play structural roles in maintaining viscoelasticity through the large number of immobilized sulfate groups on CS/DS chains. CS/DS chains also bind protein families including growth factors and growth factor receptors. Through such interactions. CS/DS chains play important roles in neurobiochemical processes, connective tissue homeostasis, coagulation, and cell growth regulation. Expression of DS has been observed to increase in cancerous tissue relative to controls. In earlier studies. MS2 was used to compare the types of CS/DS isomers present in biological samples. The results demonstrated that product ion abundances reflect the types of CS/DS repeats present and can be used quantitatively. It was not clear, however, to which of the CS/DS repeats the product ions abundances were sensitive. The present work explores the utility of MS3 for structural characterization of CS/DS oligosaccharides. The data show that MS3 product ion abundances correlate with the presence of DS-like repeats in specific positions on the oligosaccharide chains. (C) 2010 Elsevier B.V. All rights reserved.
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