Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 297, Issue 1-3, Pages 96-101Publisher
ELSEVIER
DOI: 10.1016/j.ijms.2010.06.031
Keywords
Infrared multiple photon dissociation; Fourier transform ion cyclotron resonance mass spectrometry; Carbohydrate; Isomeric differentiation; Disaccharide
Funding
- University of Florida
- National Science Foundation [CHE-0718007, OISE-0730072]
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Lithiated glucose-containing disaccharides with various linkages and anomeric configurations, formed by electrospray ionization (ESI) and trapped in a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer, were fragmented by infrared radiation from a tunable CO2 laser. Irradiation over the wavelength range from 9.2 to 9.7 mu m gave unique fragmentation patterns for each of the disaccharides. These fragmentation patterns can be used to easily determine the monosaccharide linkage position for each disaccharide. Once the linkage is determined, irradiation of the precursor ion (m/z 349) to produce a specific ratio of peak height for a particular fragment ion to that of the precursor ion is shown to yield unique ratio values that can be used to identify the different anomers of the same linkage at the 95% confidence level. (C) 2010 Elsevier B.V. All rights reserved.
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