Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 78, Issue 9, Pages 771-776Publisher
WILEY
DOI: 10.1002/jemt.22538
Keywords
dehydration; Kinorhyncha; meiofauna basket; Nematoda; processing container; SEM
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An easy and low-cost method to elaborate a container to dehydrate nematodes and other meiofauna in order to process them for scanning electron microscopy (SEM) is presented. Illustrations of its elaboration, step by step, are included. In addition, a brief methodology to process meiofauna, especially nematodes and kinorhynchs, and illustrations are provided. With this methodology it is possible to easily introduce the specimens, to lock them in a closed chamber allowing the infiltration of fluids and gases (ethanol, acetone, carbon dioxide) but avoiding losing the specimens. After using this meiofauna basket for SEM the results are efficient. Examples of nematode and kinorhynch SEM pictures obtained using this methodology are also included. Microsc. Res. Tech. 78:771-776, 2015. (c) 2015 Wiley Periodicals, Inc.
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