Journal
MICROPOROUS AND MESOPOROUS MATERIALS
Volume 204, Issue -, Pages 91-98Publisher
ELSEVIER
DOI: 10.1016/j.micromeso.2014.11.007
Keywords
Mesoporous silica nanoparticles; CpG oligodeoxynucleotides; Gene delivery; Toll-like receptor 9
Categories
Funding
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning
- National Natural Science Foundation of China [51102166]
- Program for New Century Excellent Talent in University [NCET-12-1053]
- Key Project of Chinese Ministry of Education [212055]
- Innovation Program of Shanghai Municipal Education Commission [12ZZ140]
- Shanghai Shuguang Project [12SG39]
- Hujiang Foundation of China [B14006]
- Training Program of University of Shanghai for Science and Technology [14XPY01]
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We developed a potential cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) delivery system by binding of CpG ODN onto aminated mesoporous silica nanoparticles (MSNs) to form CpG/MSN-NH2 complexes for Toll-like receptor 9 (TLR9)-mediated induction of cytokines. Serum stability, in vitro cytotoxicity, cellular uptake, and interleukin-6 (IL-6) induction of CpG/MSN-NH2 complexes were investigated. The results showed that MSN-NH2 nanopartides had no cytotoxicity to Raw 264.7 cells, and binding of CpG ODN to MSN-NH2 nanoparticles enhanced serum stability of CpG ODN due to the protection by nanoparticles. Furthermore, CpG/MSN-NH2 complexes could be efficiently taken up by cells due to small particle size and good dispersity. Most importantly, CpG/MSN-NH2 complexes significantly enhanced the level of IL-6 induction, stimulated by interaction between CpG ODN and TLR9 in endolysosomes. Therefore, MSNs would be a promising carrier for enhancing the delivery efficiency of CpG ODN. (C) 2014 Elsevier Inc. All rights reserved.
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