Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 39, Issue 14, Pages 7496-7507Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2013.08.100
Keywords
Central composite design; Response surface methodology; Distillery effluent; Enterobacter cloacae IIT-BT 08; Biohydrogen
Categories
Funding
- Ministry of New and Renewable Energy, Government of India
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Distillery effluent poses severe environmental pollution problem mainly due to its high organic content. During alcohol fermentation, most of the essential macro- and micronutrients get utilized. Therefore, supplementation of these nutrients becomes imperative for the improvement of biohydrogen production. In the present study, starch based distillery effluent was used for dark fermentative hydrogen production using Enterobacter cloacae IIT-BT 08. Hence, this study was undertaken to evaluate the effect of supplementation of yeast extract, malt extract, Fe++, Cu++ and Mg++ on biohydrogen production. The interaction among supplements and their mutual effect on the hydrogen production was studied using five factor five level central composite design). Optimum hydrogen yield of 7.4 mol H-2/kg CODreducead was predicted by the model, which showed an excellent correlation with experimental hydrogen yield of 7.38 +/- 0.24 mol H-2/kg CODreduced. An average hydrogen production rate of 80 mL/L h was achieved after supplementation, having 2.2 times higher hydrogen yield as compared to non-supplemented distillery effluent. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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