Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 39, Issue 17, Pages 8983-8991Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2014.03.171
Keywords
Cyanobacteria; Hydrogenase; Nitrogenase; Southern hybridization
Categories
Funding
- Academy of Finland [118637, 267409, 263667]
- Kone Foundation
- Nordic Energy Research, AquaFEED Project
- Academy of Finland (AKA) [263667, 263667] Funding Source: Academy of Finland (AKA)
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The genetic background and activities of the enzymes involved in H-2 production were investigated from ten distinct H-2 producing cyanobacteria, revealed by a recent screening. All strains are N-2-fixing, filamentous and heterocystous. Southern hybridization revealed that the tested strains possess the genes encoding the conventional nitrogenase (nifHDK1), and lack the alternative nitrogenases. The high H-2 production rate of these strains was shown not to be dependent on the presence of highly active nitrogenase or bidirectional hydrogenase enzymes. Moreover, most of the strains possessed a highly active uptake hydrogenase enzyme. We also examined the structure of the nif and hup operons encoding nitrogenase and uptake hydrogenase enzymes in the Calothrix 336/3 strain, the best H-2 producer in the screening. We concluded that the ability of the cyanobacteria to produce high levels of H-2 is not directly linked to the maximum capacities of the enzymes involved in H-2 production. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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