Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 37, Issue 18, Pages 13806-13814Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2012.03.151
Keywords
Biohydrogen; Palm oil mill effluent; Optimization; Thermotolerant consortia; Microbial community
Categories
Funding
- Office of the Higher Education Commission, Thailand
- Research Group for the Development of Microbial Hydrogen Production Processes from Biomass, Office of the Higher Education Commission
- Graduate School, Prince of Songkla University
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Thermotolerant consortia were obtained by heat-shock treatment on seed sludge from palm oil mill. Effect of the initial pH (4.5-6.5) on fermentative hydrogen production palm oil mill effluent (POME) showed the optimum pH at 6.0, with the maximum hydrogen production potential of 702.52 rnL/L-POME, production rate of 74.54 mL/L/h. Nutrients optimization was investigated by response surface methodology with central composite design (CCD). The optimum nutrients contained 0.25 g urea/L, 0.02 g Na2HPO4/L and 0.36 g FeSO4 center dot 7H(2)O/L, giving the predicted value of hydrogen production of 1075 mL/L-POME. Validation experiment revealed the actual hydrogen production of 968 mL/L-POME. Studies on the effect of temperature (25-55 degrees C) revealed that the maximum hydrogen production potential (985.3 mL/L-POME), hydrogen production rate (75.99 mL/L/h) and hydrogen yield (27.09 mL/g COD), were achieved at 55, 45 and 37 degrees C, respectively. Corresponding microbial community determined by the DGGE profile demonstrated that Clostridium spp. was the dominant species. Clostridium paraputrificum was the only dominant bacterium presented in all temperatures tested, indicating that the strain was thermotolerant. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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