Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 36, Issue 9, Pages 5280-5288Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2011.01.029
Keywords
Biohydrogen; Microbial community analysis; Mixed culture; DGGE; Quantitative PCR; Melting curve
Categories
Funding
- Academy of Finland [107425, 126974]
- Nordic Energy Research [28-02]
- Tampere University of Technology Graduate School
- Foundation of Research of Natural Resources in Finland
- Academy of Finland (AKA) [107425, 107425] Funding Source: Academy of Finland (AKA)
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Molecular methods, both qualitative and quantitative, have become important in hydrogen-fermenting community analyses to reveal the complexity of the community and to monitor the changes and interactions in the communities during bioprocesses. This review presents a survey of the methods used to characterize hydrogen-fermenting mixed cultures. Each method has its pros and cons and, therefore, several methods are often used in combinations to provide the best results. The methods used are mainly PCR based targeting the 16S rRNA gene, but also other methods and targets, such as the hydrogenase genes or their transcripts, are well represented. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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