Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 33, Issue 9, Pages 2409-2412Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2008.02.054
Keywords
formate detection; potassium permanganate; Escherichia coli; formate hydrogen lyase
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Mutagenesis of Escherichia coli for hydrogen production is difficult since there is no high-throughput screen. Here we describe a method for rapid detection of enhanced hydrogen production by engineered strains by detecting formate via potassium permanganate; in E. coli, hydrogen is synthesized from formate using the formate hydrogen lyase system. (c) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
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