Journal
INTERNATIONAL JOURNAL OF HEMATOLOGY
Volume 92, Issue 1, Pages 129-135Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s12185-010-0626-5
Keywords
miRNA; Acute promyelocytic leukemia; TGF beta/Smad signal transduction pathway; Smad4
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Funding
- Shanghai Municipal Commission of Sciences and Technology [09ZR1418400]
- Shanghai Municipal Education Commission [06BZ031]
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The expression pattern of microRNAs (miRNAs) and their potential target genes were investigated in acute promyelocytic leukemia (APL) cell line NB4 cells during all-trans-retinoid acid (ATRA) treatment by using a miRNA microarrays platform and real-time quantitative PCR (RTQ-PCR). MiR-146a as one of the miRNAs down-regulated by ATRA during APL differentiation was identified. Direct interaction between miR146a and its predictive target gene Smad4 were confirmed by Luciferase assay. Down-regulation of miR-146a and upregulation of Smad4 at protein levels were demonstrated. These data suggested that miR-146a might influence proliferation of APL cells through TGF-beta 1/Smad signal transduction pathway during ATRA induction.
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