Journal
MICROCHIMICA ACTA
Volume 182, Issue 15-16, Pages 2443-2450Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1592-7
Keywords
Carbon dots; Fluorescence resonance energy transfer; Quercetin; Zn(II) detection; HeLa cells
Categories
Funding
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- National Basic Research Program of China (973 Program) [2012CB825803, 2013CB932702]
- National Natural Science Foundation of China [51422207, 51132006, 21471106]
- Specialized Research Fund for the Doctoral Program of Higher Education [20123201110018]
- Suzhou Planning Project of Science and Technology [ZXG2012028]
- Natural Science Foundation of Jiangsu Province of China [BK20140310]
- China Postdoctoral Science Foundation [2014M560445]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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We describe the preparation of carbon dots (CDs) from glucose that possess high stability, a quantum yield of 0.32, and low toxicity (according to an MTT assay). They were used, in combination with the fluorogenic zinc(II) probe quercetin to establish a fluorescence resonance energy transfer (FRET) system for the determination of Zn(II). The CDs are acting as the donor, and the quercetin-Zn(II) complex as the acceptor. This is possible because of the strong overlap between the fluorescence spectrum of CDs and the absorption spectrum of the complex. The method enables Zn(II) to be determined in the 2 to 100 mu M concentration range, with a 2 mu M detection limit. The method was applied to image the distribution of Zn(II) ions in HeLa cells.
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