Journal
MICROCHIMICA ACTA
Volume 182, Issue 9-10, Pages 1733-1738Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1506-8
Keywords
Photometry; Peroxidase mimic; Hydrogen peroxide; Copper complex; Enzymatic biosensor
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Funding
- National Natural Science Foundation of China [21375079, 51402175]
- Project of Development of Science and Technology of Shandong Province, China [2013GZX20109]
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A nanomaterial of the chemical composition Cu-2(OH)(3)Cl-CeO2 and with a large surface area is shown to be a viable peroxidase mimetic. It was synthesized by co-precipitation of an aqueous solution containing Ce(III) chloride, Cu(II) chloride and hexamethylenetetramine by adding an ionic liquid. The material was characterized by scanning electron microscopy and X-ray powder diffractometry. The composite possesses peroxidase-like activity and catalyzes the oxidation of the peroxidase substrate 3,3',5,5'-tetramethylbenzidine by H2O2 to produce a blue product. Based on this finding, a simple, rapid and selective colorimetric method was worked out for the determination of glucose and cholesterol by using the respective oxidases and by quantifying the H2O2 formed. Both glucose and cholesterol can be determined by this method at levels as low as 50 A mu M.
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