Journal
MICROCHIMICA ACTA
Volume 182, Issue 9-10, Pages 1653-1660Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1481-0
Keywords
Upconversion luminescence; Downconversion luminescence; Terbium complex; Mesoporous anocomposite; Bioimaging; Temperature sensing
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Funding
- National Natural Science Foundation of China [21231004, 21201117]
- Innovation Program of Shanghai Municipal Education Commission [13ZZ073]
- Science and Technology Commission of Shanghai Municipality [13NM1401100, 13NM1401101, 14520722200]
- Shanghai Rising-Star Program [14QA1401800]
- State Key Laboratory of Rare Earth Resource Utilization [RERU2014012]
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A new multifunctional nanocomposite is described that displays both upconversion and downconversion luminescence. It is based on upconversion nanoparticles (UCNPs) with a mesoporous coating that is boned with a terbium(III) complex. The modified mesoporous nanocomposite was characterized by transmission electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherms, and photoluminescence spectra. They display dual (green and red) fluorescence under 980-nm excitation, and green fluorescence under 365-nm excitation. Both emissions can be easily detected by bare eyes. The material has low cytotoxicity and good biocompatibility as proven by the methyl thiazolyltetrazolium (MTT) assay. The nanocomposite was successfully applied to upconversion luminescence based in-vitro confocal imaging of the cytosol of murine macrophage cells (RAW264.7), and this resulted in images of excellent contrast. In addition, the particles display strongly temperature-dependent luminescence in the range from 280 K to 330 K.
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